Pressurized liquid extraction of bioactive compounds from grape peel and application in pH-sensing carboxymethyl cellulose films: A promising material to monitor the freshness of pork and milk.

This study produced pH-sensing carboxymethyl cellulose (CMC) films functionalized with bioactive compounds obtained by pressurized liquid extraction (PLE) of grape peel to monitor the freshness of pork and milk. A semi-continuous PLE was conducted using hydroethanolic solution (70:30, v/v) at a flow rate of 5 mL/min, 15 MPa, and 60 °C. The films were produced by the casting technique using CMC (2.5 %, w/v), glycerol (1 %, v/v), and functionalized with 10, 30, and 50 % (v/v) grape peel extract. From the results obtained, LC-MS/MS revealed that PLE extracted twenty-seven phenolic compounds. The main phenolic compounds were kaempferol-3-glucoside (367.23 ± 25.88 µg/mL), prunin (270.23 ± 3.62 µg/mL), p-coumaric acid (236.43 ± 26.02 µg/mL), and procyanidin B1 (117.17 ± 7.29 µg/mL). The CMC films presented suitable color and mechanical properties for food packaging applications. The addition of grape peel extract promoted the pH-sensing property, showing the sensitivity of anthocyanins to pH changes. The films functionalized with grape peel extract presented good release control of bioactive compounds, making them suitable for food packaging applications. When applied to monitor the freshness of pork and milk, the films exhibited remarkable color changes associated with the pH of the food during storage. In conclusion, PLE is a sustainable approach to obtaining bioactive compounds from the grape peel, which can be applied in the formulation of pH-sensing films as a promising sustainable material to monitor food freshness during storage.

Application of nanoparticles entrapped orange essential oil to inhibit the incidence of phytopathogenic fungi during storage of agroecological maize seeds.

The demand for sustainable, healthy, and pesticide-free food has grown in recent years. Agroecological seeds cannot receive chemical treatment, as pesticides present toxicological and environmental risks, requiring the development of alternative methods for disease control, such as the use of essential oils. In this study, orange essential oil was extracted and encapsulated in zein nanoparticles by the nanoprecipitation method. The nanoparticles were tested for the antifungal activity on agroecological maize seeds and for the mycelial sensitivity of Stenocarpella macrospora. The synthesized nanoparticles presented good encapsulation efficiency (99 %) of orange essential oil rich in D-limonene, conferring high antioxidant activity to the loaded nanoparticles. The release profile indicated a pseudo-Fickian mechanism governed by diffusion, explained according to the Korsmeyer-Peppas model. The dynamic light scattering, and transmission electron microscopy showed spherical nanoparticles with particle size lower than 200 nm. The nanoparticles containing orange essential oil inhibited the incidence of Fusarium during the storage of agroecological maize seeds. The mycelial sensitivity against Stenocarpella macrospora showed that the encapsulated essential oil was more effective in inhibiting the fungus when compared to the non-encapsulated oil. Therefore, the nanoparticles containing encapsulated orange essential oil can be effectively applied as an antifungal material for the conservation of agroecological maize seeds, contributing to the development of sustainable agricultural biotechnology with pesticide-free products.

Sustainable valorization of pitaya (Hylocereus spp.) peel in a semi-continuous high-pressure hydrothermal process to recover value-added products.

This study evaluated the use of a semi-continuous high-pressure hydrothermal process for the recovery of value-added products from pitaya peel. The process was carried out at 15 MPa, a water flow rate of 2 mL/min, a solvent-to-feed ratio of 60 g water/g pitaya peel, and temperatures ranging from 40 to 210 °C. The results show that extraction temperatures (between 40 and 80 °C) promoted the recovery of betacyanin (1.52 mg/g), malic acid (25.6 mg/g), and citric acid (25.98 mg/g). The major phenolic compounds obtained were p-coumaric acid (144.63 ± 0.42 µg/g), protocatechuic acid (91.43 ± 0.32 µg/g), and piperonylic acid (74.2 ± 0.31 µg/g). The hydrolysis temperatures (between 150 and 210 °C) could produce sugars (18.09 mg/g). However, the hydrolysis process at temperatures above 180 °C generated Maillard reaction products, which increased the total phenolic compounds and antioxidant activity of the hydrolysates. Finally, the use of semi-continuous high-pressure hydrothermal process can be a sustainable and promising approach for the recovery of value-added compounds from pitaya peel, advocating a circular economy approach in the agri-food industry.

Techno-economic analysis of single-stage and temperature-phase anaerobic co-digestion of sewage sludge, wine vinasse, and poultry manure.

Anaerobic co-digestion (AcoD) is a mature and consolidated waste management technology that can transform agro-industrial by-products into biogas and digestate. This study conducted a techno-economic assessment of bioenergy and agricultural fertilizer production from AcoD of sewage sludge, wine vinasse, and poultry manure. In this case study, three configurations were investigated: i) Scenario 1, AcoD in thermophilic temperature; ii) Scenario 2, AcoD in mesophilic temperature; and iii) Scenario 3, AcoD in a temperature phase (TPAD) system, where the digestate produced in the first reactor (thermophilic) feeds the second reactor (mesophilic). The process was designed to manage 24,022 m³ wine vinasse y-1, 24,022 m³ sewage sludge y-1, and 480 m³ poultry manure y-1. The major cost was the fixed capital investment for the single-stage (320,981 USD) and TPAD processes (379,698 USD). The TPAD process produced the highest electricity (1058.99 MWh y-1) and heat (4765.47 GJ y-1) with the lowest cost of manufacturing for electricity (84.99 USD MWh-1), heat (0.019 USD MJ-1), and fertilizer (30.91 USD t-1). Regarding the profitability indicators, the highest net present value (509,011 USD) and the lowest payback time (4.24 y) were achieved for Scenario 3. In conclusion, TPAD is a profitable and sustainable waste-to-energy management technology that can be applied in a circular economy framework to recover bioenergy and fertilizer, contributing to decreasing the carbon footprint of the agri-food sector.

Advances in process design, techno-economic assessment and environmental aspects for hydrothermal pretreatment in the fractionation of biomass under biorefinery concept.

The development and sustainability of second-generation biorefineries are essential for the production of high added value compounds and biofuels and their application at the industrial level. Pretreatment is one of the most critical stages in biomass processing. In this specific case, hydrothermal pretreatments (liquid hot water [LHW] and steam explosion [SE]) are considered the most promising process for the fractionation, hydrolysis and structural modifications of biomass. This review focuses on architecture of the plant cell wall and composition, fundamentals of hydrothermal pretreatment, process design integration, the techno-economic parameters of the solubilization of lignocellulosic biomass (LCB) focused on the operational costs for large-scale process implementation and the global manufacturing cost. In addition, profitability indicators are evaluated between the value-added products generated during hydrothermal pretreatment, advocating a biorefinery implementation in a circular economy framework. In addition, this review includes an analysis of environmental aspects of sustainability involved in hydrothermal pretreatments.

Subcritical water pretreatment enhanced methane-rich biogas production from the anaerobic digestion of brewer's spent grains.

ABSTRACTThis study evaluated the effectiveness of a semi-continuous flow-through subcritical water hydrolysis (SWH) pretreatment of brewer's spent grains (BSG) for subsequent application in the anaerobic digestion (AD) process. BSG pretreatment was conducted at 160 °C and 15 MPa with a flow rate of 10 mL water min-1 and 15 g water g-1 BSG. The results revealed that SWH attacked the hemicellulose structure, releasing arabinose (46.54 mg g-1) and xylose (39.90 mg g-1) sugars, and proteins (34.89 mg g-1). The start-up of anaerobic reactors using pretreated BSG (747.71 L CH4 kg-1 TVS) increased the methane yield compared with the reactor without pretreatment (53.21 L CH4 kg-1 TVS). For the process with pretreatment, the generation of electricity (134 kWh t-1 BSG) and heat (604 MJ t-1) are responsible for the mitigation of 43.90 kg CO2 eq t-1 BSG. The adoption of SWH as an eco-friendly pretreatment of biomass for AD could be a technological route to increase methane-rich biogas and bioenergy production, supporting the circular economy transition by reducing the carbon footprint of the beer industry.

Pressurized liquid extraction coupled in-line with SPE and on-line with HPLC (PLE-SPExHPLC) for the recovery and purification of anthocyanins from SC-CO2 semi-defatted Açaí (Euterpe oleracea).

This study aimed to extract anthocyanins from dried and semi-defatted açaí pulp using green technologies based on the coupling of pressurized liquid extraction (PLE) with in-line purification through solid-phase extraction (SPE) and on-line analysis by high-performance liquid chromatography (HPLC). Critical parameters that affect the extraction efficiency and purification were investigated and optimized by response surface methodology (RSM). PLE was performed with acidified water at different pH (2.0, 4.5, and 7.0) and temperatures (40, 80, and 120 °C) at 15 MPa, 2 mL/min, and solvent-to-feed mass ratio equal to 40. SPE was optimized in a column packed with the adsorbent PoraPak™ Rxn. Different ethanol concentrations (50, 75, and 100 %) and temperatures (30, 40, and 50 °C) were evaluated for the anthocyanin's elution. The optimal conditions of the two experimental designs were determined by the RSM, firstly for PLE: 71 °C and pH 2; then using this PLE condition, the optimization of the SPE was obtained: 30 °C and 50 % ethanol. The developed PLE method provided similar anthocyanin yield to other techniques, and the coupling with SPE in-line produced an extract 5-fold more concentrated than PLE alone. Therefore, the system (PLE-SPE × HPLC-PDA) proved to be a powerful tool for monitoring the extraction process in real-time.

Production of methylcellulose films functionalized with poly-ε-caprolactone nanocapsules entrapped ß-carotene for food packaging application.

Nowadays, there is a worldwide demand in the production of innovative packaging that release active compounds to increase the shelf life of perishable food products. Therefore, this study produced methylcellulose films functionalized with poly-ε-caprolactone nanocapsules entrapped ß-carotene. The nanoparticles were produced by the nanoprecipitation method, and 10, 30, and 50 % of nanoparticles colloidal solution was added in the methylcellulose filmogenic solution. The films were characterized by the mechanical, physicochemical properties, antioxidant activity, and release of ß-carotene from the polymeric matrix to a food simulant. The results demonstrated satisfactory mechanical properties; however, the addition of nanoparticles decreased the Young's Modulus and increased the elongation at break. Regarding light transmission, the incorporation of ß-carotene nanoparticles promoted a decrease in the percentage of ultraviolet ray's transmittance through the film matrix, as well as visible light. The incorporation of nanoparticles improved the antioxidant activity of the films, which was proportional to the concentration of ß-carotene used in the formulation. The release of ß-carotene reached a maximum value of 10.93 µg g-1 film containing 70 % nanoparticles, which was a desired profile for food application. Finally, the methylcellulose films functionalized with poly-ε-caprolactone nanocapsules can release ß-carotene, and therefore, can be considered as a novel nanomaterial for food conservation, with a potential to increase the shelf life of perishable food products.

Recovery of sugars and amino acids from brewers' spent grains using subcritical water hydrolysis in a single and two sequential semi-continuous flow-through reactors.

This study evaluated the subcritical water hydrolysis (SWH) of brewer's spent grains (BSG) to obtain sugars and amino acids. The experimental conditions investigated the hydrolysis of BSG in a single flow-through reactor and in two sequential reactors operated in semi-continuous mode. The hydrolysis experiments were carried out for 120 min at 15 MPa, 5 mL water min-1, at different temperatures (80 - 180 °C) and using an S/F of 20 and 10 g solvent g-1 BSG, for the single and two sequential reactors, respectively. The highest monosaccharide yields were obtained at 180 °C in a single reactor (47.76 mg g-1 carbohydrates). With these operational conditions, the hydrolysate presented xylose (0.477 mg mL-1) and arabinose (1.039 mg mL-1) as main sugars, while low contents of furfural (310.7 µg mL-1), 5-hydroxymethylfurfural (<1 mg L-1), and organic acids (0.343 mg mL-1) were obtained. The yield of proteins at 180 °C in a process with a single reactor was 43.62 mg amino acids g-1 proteins, where tryptophan (215.55 µg mL-1), aspartic acid (123.35 µg mL-1), valine (64.35 µg mL-1), lysine (16.55 µg mL-1), and glycine (16.1 µg mL-1) were the main amino acids recovered in the hydrolysate. In conclusion, SWH pretreatment is a promising technology to recover bio-based compounds from BSG; however, further studies are still needed to increase the yield of bioproducts from lignocellulosic biomass to explore two sequential reactors.

Research Trends in the Study of Edible Mushrooms: Nutritional Properties and Health Benefits.

The consumption of edible-culinary mushrooms for the prevention and treatment of chronic disease has gained increasing attention. This review summarizes trends in the biotechnological and medicinal potential of edible mushrooms cultivated worldwide. Macronutrients (fatty acids, amino acids, and carbohydrates), bioactive compounds (phenolics, flavonoids, and carotenoids), and health benefits (antioxidant, antimicrobial, antifungal, anticancer, and pre-biotics properties) of mushrooms are described, including their cultivation, industrial processing, and consumption. In general, edible-culinary mushrooms present a rich nutritional composition with beneficial properties for human health. Indeed, the consumption of edible mushrooms is associated with a reduction in the risk of cancer and diabetes. Furthermore, mushrooms can be incorporated into different food formulations and used as a medicinal substance due to their mycochemicals with antioxidant capacity. Edible mushrooms are considered a "superfood" and can be recommended as a valuable constituent in the daily diet. In conclusion, this review describes trends, future decision-making, and guidance on the health benefits of edible mushrooms.

Application in situ of biodegradable films produced with starch, citric pectin and functionalized with feijoa (Acca sellowiana (Berg) Burret) extracts: An effective proposal for food conservation.

In this study, biodegradable films produced with starch, citric pectin, and functionalized with antioxidant compounds from feijoa (Acca sellowiana (Berg) Burret) were in situ applied for the conservation of ground beef, bread, and grapes. The results demonstrated that the films produced were an excellent source of stable antioxidant compounds, with antimicrobial activity against Escherichia coli, Salmonella, and Shigella. The bioactive films based on biological macromolecules positively stabilized the polyunsaturated fatty acids and deterioration reactions in ground beef. The release of bioactive compounds from the films was responsible for inhibiting molds and yeasts in bread, increasing their shelf life for 30 days of storage. The application of film coating and packaging in grapes increased postharvest conservation and maintained steady physicochemical characteristics. Therefore, the innovative films produced can release bioactive compounds with antioxidant and antimicrobial activity, and consequently, can be proposed as an effective material for food conservation, increasing the shelf life of perishable food products.

Chitosan packaging functionalized with Cinnamodendron dinisii essential oil loaded zein: A proposal for meat conservation.

The nanoencapsulation of essential oils for biodegradable films functionalization is a viable alternative for the production of active food packaging. In this study, the Cinnamodendron dinisii Schwanke essential oil was nanoencapsulated using zein as wall material, and applied in chitosan matrix to produce an active nanocomposite film packaging for food conservation. The chemical composition of the Cinnamodendron dinisii Schwanke essential oil showed a variety of unexplored bioactive compounds, and 1,8-cineole was the major compound. The oil nanoencapsulation produced stable and homogeneous nanoparticles with zeta potential close to 30 mV and polydispersity index lower than 0.2. The nanoparticles size showed a size variation between 70 and 110 nm. The chitosan films obtained functionalized with nanoparticles demonstrated antioxidant activity and antimicrobial activity. The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.

Feijoa [Acca sellowiana (Berg) Burret] accessions characterization and discrimination by chemometrics.

BACKGROUND: Feijoa [Acca sellowiana (Berg) Burret] is a Brazilian native fruit with few commercial-level plantations and high agroindustrial potential. A genotype evaluation experiment was conducted from 1996 onward, aiming to obtain fruits based on the agronomical parameters; however, the selection based on chemical composition had not been evaluated with respect to developing a new cultivar. Accordingly, the present study aimed to discriminate seven accessions of feijoa in terms of nutritional composition, phenolic compounds and antioxidant activity using multivariate analysis (principal component analysis and multivariate contrast), targeting the potential production of a new cultivar with better nutritional value and high antioxidant capacity. RESULTS: Feijoa husk presented high content of ashes, lipids, proteins, carbohydrates, phenolic compounds and antioxidant activity compared to feijoa pulp. However, only feijoa pulp was selected to multivariate analysis because it is the fruit edible part. Data variability was explained in 78% and the feijoa pulp accessions were discriminated into four groups related to their characteristics. The accession 5 discrimination can be explained by the high content of ashes, carbohydrates, soluble solids, phenolic compounds and antioxidant activity. Accession 6 was also discriminated by the high content of total acidity, pH and proteins, as well as a low content of soluble solids. CONCLUSION: Feijoa accessions may be indicated for increasing plant selection via hybridization with the other accessions, aiming to produce new cultivars with better nutritional composition and antioxidant capacity. For example, accession 5 is the most suited fruit for human consumption and is a potential plant with respect to becoming a new cultivar. © 2020 Society of Chemical Industry.

Bioactive food packaging based on starch, citric pectin and functionalized with Acca sellowiana waste by-product: Characterization and application in the postharvest conservation of apple.

Innovative biodegradable packaging with antimicrobial and antioxidant properties was developed, and functionalized with Acca sellowiana waste by-product (feijoa peel flour, FPF). Physicochemical, morphological, antioxidant, antimicrobial properties, and in situ application in the postharvest conservation of apple were conducted with the packaging produced. The results obtained demonstrate that FPF addition had a positive influence on the packaging characteristics, for all the parameters tested. The high concentration of antioxidant compounds in the films with FPF promoted antimicrobial activity against Escherichia coli, Salmonella typhimurium, and Pseudomonas aeruginosa. The packaging produced maintained the quality of apples during storage, with constant weight after 5 days of storage. Based on our results, the bioactive, antioxidant and antimicrobial packaging functionalized with Acca sellowiana waste by-product may be considered as a new alternative to packaging in food systems.

Investigation of Nutritional Composition, Antioxidant Compounds, and Antimicrobial Activity of Wild Culinary-Medicinal Mushrooms Boletus edulis and Lactarius deliciosus (Agaricomycetes) from Brazil.

Wild edible mushrooms are found around the world, and widely appreciated in gastronomy, medicine, and pharmacology. This study investigated the chemical characterization of Boletus edulis and Lactarius deliciosus wild edible mushrooms, naturally grown in the mountain range of Santa Catarina, Brazil. Mushrooms were separated in stem and cap, and the nutritional composition was conducted in fresh matter. Antioxidant compounds were extracted in three solvents, and total phenolic compounds, flavonoids, antioxidant capacity, and antimicrobial activity were evaluated. The results demonstrate that moisture, ashes, lipids, crude protein, carbohydrates, and calories presented a significant difference according to the species evaluated. Mushrooms presented more than 85% of moisture, high content of crude protein, and carbohydrates. B. edulis had the highest content of antioxidant compounds compared with L. deliciosus, tested by DPPH, ABTS, and FRAP assay. High free radical inhibition and low IC50 values were obtained, demonstrating that B. edulis presents a strong antioxidant capacity. Regarding the antimicrobial activity, B. edulis extracts were effective in controlling Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae, demonstrating biological application. Based on the aforementioned, future studies can be addressed in the development of innovative food products, medicines, and pharmaceuticals, using B. edulis and L. deliciosus wild edible mushroom. Therefore, wild edible mushrooms evaluated in this study had the potential to be used in human consumption, providing essential nutrients, phenolic compounds, and flavonoids, with high antioxidant and antimicrobial activity.

Development and Characterization of Poly-ε -caprolactone Nanocapsules Containing β -carotene Using the Nanoprecipitation Method and Optimized by Response Surface Methodology

Abstract Nanoparticles demonstrate an important role in the protection of bioactive compounds from external factors such as temperature, oxygen and light. In this study, poly-ε-caprolactone (PCL) nanoparticles entrapped β-carotene was produced using the nanoprecipitation method. Firstly, was evaluated the lipophilic surfactant effect and carrier agent of the active compound in the nanocapsules formulation. After choosing the most stable formulation, the nanocapsules production was optimized using β-carotene, caprylic/capric triglycerides (CCT) and soybean lecithin. Response surface methodology (RSM) was adopted to evaluate the influence of soy lecithin concentration, volume of CCT and β-carotene concentration in the particle size, zeta potential, polydispersity index (PDI), encapsulation efficiency and recovery. Formulations containing soy lecithin and CCT demonstrated better stability comparing to the other formulations tested. The nanoparticle formulations presented an optimized particle size below 200 nm, PDI lower than 0.1 and encapsulation efficiency above 95%. Based on the results obtained, the optimum conditions to prepare PCL nanocapsules were 0.2160 mg/mL of β-carotene, 232.42 μL of CCT and 2.59 mg/mL of soy lecithin, suggesting an applicability to promote controlled released of β-carotene in food system.

Geographical discrimination of uvaia (Eugenia pyriformis Cambess) by principal component analysis.

BACKGROUND: The international marketing of native Brazilian fruits may benefit from research into discrimination between plants with particular properties. Plants with certain characteristics have strong potential for use in agroindustry and for the development of new processed products. In the current study, 12 uvaia plants (Eugenia pyriformis Cambess), native to Brazil, were geographically discriminated by chemometric methods, using nutritional composition, physicochemical characterization, bioactive compounds, and antioxidant capacity data. RESULTS: Uvaia fruits presented good nutritional value, with a high moisture and carbohydrate content. All fruits presented a pH value lower than 4, being classified as highly acidic, and the total soluble solids (°Brix) ranged between 2.90 and 9.80. Some plants had a higher vitamin C content, and all the plants had a high bioactive compound content (phenolic and flavonoids) and antioxidant capacity in 1,1-difenil-2-picrilhidrazil (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assays. Hydroethanolic solution was the best extraction solvent tested when compared with water and ethanol. Using principal component analysis, it was possible to discriminate among the plants in five different groups; however, just one group was responsible for higher antioxidant compound content. CONCLUSION: In this work, 12 native uvaia plants were discriminated by their geographical origin using a chemometric approach. Genetic improvement based on natural selection could be accomplished with some of the plants to improve the quality of uvaia pulp and to develop new cultivars. © 2019 Society of Chemical Industry.